Growth factor and other nutritional requirements of the acetone butanol organism, Cl. acetobutylicum

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Abstract
2 growth factors are required by Clostridium acetobutylicum, viz. biotin and another factor, present in yeast extract, which is not identical with any known growth factor for miero-organisms, and which has been obtained in a highly concentrated form. In presence of these 2 factors the organism will grow normally and also produce solvents from glucose with ammonium phosphate as the sole source of N. There is no strict correlation between growth and fermentation, and the presence of other factors may be necessary in order that normal solvent production may take place concurrently with normal growth. Certain other sugars are also fermented in the simple ammonium phosphate medium containing the 2 growth factors.

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