An optimal growth medium for the dinoflagellate Crypthecodinium cohnii
- 6 March 1975
- journal article
- Published by Taylor & Francis in Phycologia
- Vol. 14 (1)
- https://doi.org/10.2216/i0031-8884-14-1-1.1
Abstract
A synthetic defined medium [NaCI 342 mM, MgSO428 mM, CaCl27.5 mM, KC19 mM, disodium glycerophosphate 0.79 mM, (NH4)2SO41.5 mM, sodium acetate 15 mM, l · histidine · HCl 0.8 mM, biotin 8.2 × 10−9 M, thiamin · HCl 2.96 × 10−6 M, vitamin B127.49 × 10−10 M, d-glucose 22 mM, betaine · HCl 9.7 mM, MES 8 mM, F metal mix (100 × stock solution contains: nitrilotriacetic acid 6 mM, 5-sulphosalicylic acid 0.08 mM, Fe(NH4)2(SO4)20.18 mM, NaOH 30 mM, pH to 3.8 with HCl), final medium pH=6.6] for optimal growth of the dinoflagellate, Crypthecodinium cohnii, has been experimentally defined. Optimal growth is obtained in the dark. The temperature optimum for binary fission is 27°C. The pH optimum is 6.6. Agitation of cultures is inhibitory. In this medium under these optimal conditions C. cohnii will undergo three divisions per day.Keywords
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