NADPH:cytochrome P-450(c) reductase: Biochemical characterization in rat brain and cultured neurons and evolution of activity during development
- 1 September 1989
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 14 (9) , 883-887
- https://doi.org/10.1007/bf00964819
Abstract
NADPH:cytochrome P-450 (c) reductase is a microsomal enzyme which is involved in the cytochrome P-450-dependent biotransformation of many exogenous agents as well as of some endogenous molecules. Using cytochromec as a substrate, the kinetic parameters of this enzyme were determined in brain microsomes. The comparison of the NADPH:cytochrome P-450 reductase's Vmax values and cytochrome P-450 contents in both fractions, suggests a role of cerebral NADPH:cytochrome P-450 reductase in cytochrome P-450 independent pathways. This is also supported by the different developmental pattern of brain enzyme as compared to the liver enzyme, and by the presence of a relatively high NADPH:cytochrome P-450 reductase activity in immature rat brain and neuronal cultures, while cytochrome P-450 was hardly detectable in these preparations. The enzyme activity was not induced by a phenobarbital chronic treatment neither in the adult brain nor in cultured neurons, suggesting a different regulation of the brain enzyme expression.This publication has 46 references indexed in Scilit:
- A new aspect of the protective functions of the blood-brain barrier: Activities of four drug-metabolizing enzymes in isolated rat brain microvesselsLife Sciences, 1988
- Sulphoxidation of albendazole by the FAD-containing and cytochrome P-450 dependent mono-oxygenases from pig liver microsomesXenobiotica, 1987
- Presence of NADPH-cytochrome P450 reductase in central catecholaminergic neuronesNature, 1984
- Cytochrome Reductase Activities in Rat Brain Microsomes During DevelopmentJournal of Neurochemistry, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- DIFFERENCES IN THE SUBCELLULAR AND SUBSYNAPTOSOMAL DISTRIBUTION OF THE PUTATIVE ENDOPLASMIC RETICULUM MARKERS, NADPH‐CYTOCHROME c REDUCTASE, ESTRONE SULFATE SULFOHYDROLASE AND CDP‐CHOLINE DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE IN RAT BRAINJournal of Neurochemistry, 1979
- Specific requirement of NADPH‐cytochrome c reductase for the microsomal heme oxygenase reaction yielding biliverdin IXαFEBS Letters, 1979
- Cytochrome P-450 and NADPH cytochrome C reductase in rat brain: Formation of catechols and reactive catechol metabolitesBiochemical and Biophysical Research Communications, 1977
- Preparation of homogeneous NADPH-cytochrome P-450 reductase from rat liverBiochemical and Biophysical Research Communications, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970