Physical map of chromosomal and plasmid DNA comprising the genome ofLeptospira interrogans
Open Access
- 25 September 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (18) , 4857-4860
- https://doi.org/10.1093/nar/19.18.4857
Abstract
The size and physical structure of the Leptospira Interrogans genome was characterized using contourclamped homogenous electric field (CHEF) gel electrophoresis. The L. Interrogans genome is approximately 4750 kb in size and is composed of two molecular species of DNA: a 4400 kb chromosome; and a 350 kb plasmid, pLIN1. A physical map of the chromosome was constructed with the restriction enzymes Nott and Sfll. A physical map of pLIN1 was constructed with Apal, Notl, Sse8387l, SgrAl, and Smal. Both the L. Interrogans chromosome and pLIN1 are circular.Keywords
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