Enzymic reduction of 2:4-dinitrophenol by rat-tissue homogenates

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Abstract
2,4-Dintrophenol is reduced by rat-liver homogenates to 4-amino-2-nitrophenol. Some 2-amino-4-nitrophenol is obtained (about 10% of the total amine). Max. rate of reduction is attained in phosphate buffer of pH 7 at 37[degree] and in the presence of Na lactate or succinate. The reaction is completely inhibited by 5 x 10-3 [image] KCN. Rat spleen, kidney, intrascapular fat, muscle and brain also destroy 2,4-dinitrophenol. The reduction products were isolated by means of alumina chromatograms and paper-partition chromatography. 4-Amino-2-nitrophenol was identified by u.-v. spectrography. Liver homogenates can reduce the isomeric aminonitrophenols. The 2d reduction product is thought to be 2,4-diaminophenol, but has not been identified. The main excretion products of 2,4-dinitrophenol in the urine of rats were unchanged 2,4-dinitrophenol and 2-amino-4-nitrophenol.
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