Urinary Lactate Dehydrogenase Activity

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Abstract
(1) A method for measurering the total activity of urinary lactate dehydrogenase (urinary LDH) is presented. The optima of pH, substrate and Coenzyme for this enzyme were determined by measuring the decrease in optical density at 366 nm of NADH formed by reduction of pyruvate to lactate. By application of this method no urinary inhibitor of LDH was detected. (2) Urinary LDH activities were investigated in patients suffering from some severe renal diseases, including unilateral renal ischaemia. Simultaneously the urinary LDH-isozyme pattern was determined by means of zone electrophoresis in agar gel. (3) The value of this supplement to the measurement of the total enzyme activity is pointed out. Thus unilateral renal ischaemia may be associated with the relative predominance of LDH-isozyme five in the urine from the affected kidney.

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