P-NITROPHENOL CONJUGATION IN PERFUSED LIVERS FROM NORMAL AND PHENOBARBITAL-TREATED RATS - INFLUENCE OF NUTRITIONAL STATE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 23  (1) , 185-193
Abstract
Conjugation of p-nitrophenol in perfused livers from control and phenobarbital-treated rats was measured spectrally by determining influent minus effluent concentration differences of the free drug. Rates of conjugation (1.0-2.0 .mu.mol/g per h) were essentially the same in livers from fasted control or fasted phenobarbital-treated rats. In control fed livers, rates of conjugation did not differ from the fasted state. Rates of conjugation in livers from carbohydrate-rich, phenobarbital-treated rats were 6- to 10-fold greater than rates in livers from fasted phenobarbital-treated animals. Differences in rates of conjugation in phenobarbital-treated animals. Differences in rates of conjugation in phenobarbital-treated rats in different nutritional states were more closely related to carbohydrate reserves as indicated by glycogen levels than to activities of p-nitrophenyl UDP-glucuronyl transferase. Relationship to carcinogenesis is discussed.