Generation of hydrogen peroxide by brown adipose tissue mitochondria

Publisher Website
Google Scholar
Abstract
This is the first report on the generation of H2O2 by brown adipose tissue mitochondria. Flavin dehydrogenase-linked substrates like succinate, glycerol-1-phosphate, and fatty acyl CoA were good substrates for the reaction, while NAD+-linked substrates were less effective. In cold-acclimated animals the activity showed a substantial increase (2.5-fold). TheK m andV max of the reaction were considerably lower than those of the respective dehydrogenase. Metal ions, particularly Cu2+ and Fe2+ were potent inhibitors of the reaction. Nucleoside diphosphates, which were inhibitors by themselves, potentiated the inhibitory action of Fe2+ ions. In most of the properties, the H2O2 generator of brown adipose tissue mitochondria resembled that of liver mitochondria.