PRODUCTION OF MONOCLONAL-ANTIBODIES WITH HEMAGGLUTINATION-INHIBITION ACTIVITY TO THE SKALICA STRAIN FROM THE TICK-BORNE ENCEPHALITIS COMPLEX

  • 1 January 1983
    • journal article
    • research article
    • Vol. 27  (1) , 34-+
Abstract
Hybridomas secreting monolconal antibodies with haemagglutination-inhibition (HI) activity to the Skalica strain of tick-borne encephalitis (TBE) complex were prepared by the fusion of P3-NS1-Ag4-1 myeloma cell line with spleen cells of BALB/c mice immunized with the purified Skalica strain. The highest titers of monoclonal antibodies obtained from the hybridomas S-9, S-15 and S-16 ranged from 512 to 10,240, respectively; the ascitic fluid contained as many as 4.6 mg/ml of monoclonal antibodies. Its analysis by Ouchterlony''s double immunodiffusion, agarose electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed the presence of monoclonal antibodies with .mu. isotype of the H chain and .kappa. isotopye of the L chain. The specificity of the monoclonal antibodies was proved using 11 different antigens from family Togaviridae in the HI test.