Multisample Enzyme Extraction from Cultured Plant Cell Suspensions
- 1 January 1971
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 47 (1) , 38-42
- https://doi.org/10.1104/pp.47.1.38
Abstract
Mitochondria were isolated from etiolated corn shoots (Zea mays L.) that were stressed to a measured water potential. The rates of mitochondrial respiration in state III, state IV, and without phosphate or ADP on a milligram protein basis decreased as water stress increased with succinate, malatepyruvate, or reduced nicotinamide adenine dinucleotide as substrates. Coupling (as determined by respiratory control and ADP/O ratios) did not decrease with increasing water stress. At water potentials greater than −35 bars all respiration had ceased.Keywords
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