ESR Studies on the Membrane Properties of a Moderately Halophilic Bacterium

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Abstract
The lipid membrane properties of a moderately halophilic gram-negative bacterium, Pseudornonas halosaccharolytica ATCC 29423, were studied by the use of stearate spin labels, 5NS, 12NS, and 16NS, changing the temperature of ESR measurement from 15 to 50°C. The order parameter and the rotational correlation time of the spin labels incorporated into intact cell membranes of this bacterium grown at various temperatures in media containing different NaCI concentrations were calculated. The activation energy of rotational micro- viscosity was obtained from Andrade plots. At low growth temperature and low NaCl concentration in the medium, extractable lipids of this bacterium contained comparatively large amounts of unsaturated fatty acids, but as the growth temperature and NaCl concentration in the medium increased, the contents of saturated and cyclopropanoic fatty acids increased to more than half of the total fatty acids. 5NS gave the highest order parameters for the intact cells of this bacterium, while 12NS gave lower and 16NS gave the lowest results. The order parameters of 5NS, 12NS, and 16NS were completely separated, and all order parameters decreased gradually as the measuring temperature was increased. In contrast, the rotational correlation times of the intact cells with 12NS were as large as those with 5NS, while those with 16NS were distinctly smaller. Increasing NaCl concentrations in the growth medium caused an increase of the rotational correlation times, that is, stiffened the lipid bilayers. The Andrade plot for 16NS was ap proximately a straight line, whereas 5NS and 12NS gave two straight lines crossing at a tem perature near the growth temperature, indicating phase transition from solid to liquid. The microviscosity activation energies were 5–10 kcal/mol in the liquid phase and 15–25 kcal/mol in the solid phase.