Studies on heart. XX. Further effects of bovine ventricle protein (BVP) and antiarrhythmic peptide (AAP) on myocardial cells in culture.
- 1 January 1980
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 28 (11) , 3340-3346
- https://doi.org/10.1248/cpb.28.3340
Abstract
To elucidate the physiological significance of bovine ventricle protein (BVP) and antiarrhythmic peptide (AAP) obtained from bovine heart, their effects on spreading, beating, arrhythmic movements, macromolecular synthesis, continuous cultivation and Ca incorporation of cultured rat myocardial cells were investigated in Eagle''s minimum essential medium (MEM) supplemented with or without serum. BVP and AAP at 10-7 M stimulated the spreading phenomenon and the protein synthesis of myocardial cells in serum-free culture, and promoted prolonged survival with spontaneous beating in culture with 1% serum. As regards the beating properties of myocardial cells, they had different effects. AAP, which improved the arrhythmic movements induced by low K, high Ca or addition of ouabain, depressed the beating rate and the ratio of beating cells at 10-7-10-6 M. BVP promoted beating in continuous cultivation over 10 days, but did not affect the arrhythmic movements induced by low K or high Ca at 10-7-10-6 M. When myocardial cells were cultured in modified Eagle''s MEM-0.5% bovine serum albumin at 0.5 mM K, 5 mM Ca or 0.2 mM ouabain (which induced arrhythmic movements of cultured cells) their spreading was significantly suppressed. Quinidine, oxytocin and insulin, which improve arrhythmia in the intact heart, stimulated the spreading phenomena and improved the arrhythmic movements induced by low K and AAP. AAP decreased the incorporation of 45Ca into myocardial cells but BVP did not have this effect. AAP shows antiarrhythmic action apparently as a result of depressions of Ca incorporation and K effusion, with low excitation and prolonged survival of myocardial cells. BVP shows prolonged survival with spontaneous and strong beating in continuous culture for 10 days.This publication has 0 references indexed in Scilit: