Trypsin-Mediated Activation of the -Haemolysin of Staphylococcus Aureus
- 1 February 1975
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 8 (1) , 29-38
- https://doi.org/10.1099/00222615-8-1-29
Abstract
Alpha protoxin of StaphyZococcus aureus “Wood 46” was activated by trypsin which had been coupled to carboxymethylcellulose, as indicated by the toxin's ability to hydrolyse tosyl-arginine methylester (TAME). A Lineweaver-Burk plot of the degradation of TAME by toxin and trypsin showed that toxin had a greater affinity for the substrate than had trypsin. N-terminal aminoacid analyses of activated toxin suggested that leucine or isoleucine is the N-terminus, in contrast to protoxin, the N-terminus of which is histidine.Keywords
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