Bombesin stimulates insulin secretion by a pancreatic islet cell line.

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Abstract
The amphibian tetradecapeptide, bombesin (BBS), stimulates insulin secretion both in vivo and by pancreatic islet cells in vitro. To determine whether BBS can act directly on pancreatic .beta. cells, its effects on insulin secretion by HIT-T15 cells (HIT cells), a clonal [hamster] islet cell line was examined. Addition of 100 nM BBS to HIT cells stimulated insulin release 25-fold within 30 s. The rapid stimulatory effect of BBS on insulin release was short-lived: the secretory rate returned to basal levels after 90 min of BBS treatment. The decrease in the rate of insulin release in the continued presence of BBS was due not to depletion of intracellular insulin stores but to specific desensitization to this peptide. Stimulation of insulin secretion by BBS was dose-dependent with an ED50 value (0.51 .+-. 0.15 nM) similar to the concentration of BBS-like immunoreactive material in rat plasma. Five BBS analogs, including porcine gastrin-releasing peptide, were as powerful as BBS in stimulating insulin release. The relative potencies of the analogs tested indicated that the COOH-terminal octapeptide sequence in BBS was sufficient for stimulation of release. In contrast, 14 peptides structurally unrelated to BBS did not alter insulin secretion. BBS action was synergistic with that of glucagon; insulin secretion in the presence of maximal concentrations of both peptides was greater than the additive effects of the 2 peptides added individually. Somatostatin inhibited BBS-stimulated release by 69 .+-. 1% with an ID50 value of 3.2 .+-. 0.3 nM. BBS stimulation of insulin secretion by a clonal pancreatic cell line closely parallels its effects in vivo. BBS apparently stimulates insulin secretion by a direct effect on the pancreatic .beta. cell. The clonal HIT cell line provides a homogeneous cell preparation amenable for studies on the biochemical mechanisms of BBS action in the endocrine pancreas.