Role of Pancreatic Juice in Cholesterol Absorption

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Abstract
Pancreatic juice and bile were excluded from the intestinal tract of rats by cannulating the common bile duct at its duodenal junction. As judged by the recovery of the C14 of administered C14-cholesterol in thoracic duct lymph, this preparation has lost the capacity for absorbing cholesterol. Continuous administration of pancreatic juice alone does not restore cholesterol absorption. The continuous administration of bile alone enables the test rat to absorb cholesterol, but slower than normal. In the presence of bile, pancreatic juice stimulates cholesterol absorption. Normal cholesterol absorption was obtained by the continuous dripping of both bile and pancreatic juice into the duodenums of the test rats. Homog-enates prepared from the duodenums of rats in which pancreatic juice was prevented from entering the intestinal tract gradually lose the capacity for esterifying cholesterol, and after 24 hours the loss is almost complete. At this time the rat has also lost the ability to absorb cholesterol.