Contribution of Azospirillum brasilense Cd to growth of tomato seedlings is not through nitrogen fixation

Abstract
A wild type and a site-directed mutant strain (Nif) of Azospirillum brasilense Cd, totally deficient in N2-fixation capability, were used to evaluate if improvement in tomato seedling growth is due to the N2-fixation process. The mutant (Nif) exhibited several physiological, ecological, and antigenic features similar to the parental strain. When inoculated onto tomato seeds, the significant positive effects of the A. brasilense Nif on seedling growth under greenhouse conditions in artificial medium were similar to that of the wild-type strain. It is concluded that the contribution of A. brasilense Cd to the improvement of tomato seedling growth is not through N2-fixation.