Effect of Methylcholanthrene or Phenobarbital Pretreatment of Rabbits and Rats on the Extinction Coefficient for Cytochrome P-450

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Abstract
Phenobarbital (PB) or methylcholanthrene (3-MC) pretreatment of rats or rabbits causes induction of cytochrome P-450. In rabbits neither pretreatmentchanges the extinction coefficient for cytochrome P-450-CO complex when this is estimated from the amount of totalheme, cytochrome b5 and absorbancy at 450 nm. In rats PB pretreatment does not change the extinction coefficient, while 3-MC causes an increase in the average extinction coefficient from 85 to 102 mM–1cm–1.