HOG CHOLERA V DEMONSTRATION OF ANTIGEN IN SWINE TISSUES BY FLUORESCENT ANTIBODY TECHNIQUE

  • 1 January 1965
    • journal article
    • research article
    • Vol. 29  (12) , 299-+
Abstract
The fluorescent antibody technique was employed to detect hog cholera virus in tissue sections of various organs from experimentally infected swine. The method proved to be highly sensitive and infection could be detected in these animals as early as 3 days after inoculation with the virus. Best results were obtained when tissues were collected from young animals in advanced disease rather than from sows or from pigs in the early febrile phase. Tonsil, spleen and lymph node were the tissues of choice and were most satisfactory when removed from freshly killed animals rather than from those that had died.