Combined Fluorometer and Double-Beam Spectrophotometer for Reflectance Measurements

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Abstract
An apparatus suitable for recordings of extracellular oxygen tension in terms of the degree of hemoglobin dissociation and intracellular oxidation‐reduction state in terms of the fluorescence level of mitochondrial and cytoplasmic reduced pyridine nucleotide has been constructed. A time sharing sequence permitting light and dark intervals for reflectance fluorometry and two flashes of different wavelengths for reflectance spectrophotometry operates at a repetition frequency of 60 cps. Fixed filters are used in fluorometry for excitation and emission and continuously variable interference filters are used in spectrophotometry.