RADIOIMMUNOASSAY OF SPERMIDINE IN HUMAN-SERUM

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 19  (2) , 295-309
Abstract
Specific antispermidine antibodies were produced following immunization of New Zealand white rabbits with a spermidine-thyroglobulin conjugate of high (> 200:1) hapten-carrier molar ratio. The harvested antispermidine rabbit antiserum was characterized and a radioimmunoassay [RIA] procedure developed. The specificity of this antibody was tested against cadaverine, putrescine, spermine, monoacetylputrescine, N-8-acetylspermidine and other compounds of similar molecular structure. Negligible cross-reactivity was observed with putrescine (2.15), cadaverine (0.6%), spermine (0.3%) and diaminopropane (< 0.1%), and no cross-reaction was found with monoacetylputrescine, N-8-acetylspermidine, histamine, L-lysine and L-ornithine. The spermidine values on serum of 5 healthy volunteers were determined by RIA and compared with values by gas chromatography-mass spectrometry. Statistical evaluations of the results were highly agreeable. Antibody titer, intra-assay precision and analytical accuracy were tested. The assay requires only 20 .mu.l of serum and has picomole sensitivity. The sensitivity, specificity and small sample requirement make this procedure an effective tool for spermidine analysis.

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