Improved method for the purification of Hog Cholera Virus grown in tissue culture

Publisher Website
Google Scholar
Abstract
Summary A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved by rate zonal centrifugation in a 7–35 per cent sucrose gradient. This procedure permits a recovery of approximately 40 per cent of viral infectivity. A specific infectivity of about 2×107 PFU/µg of protein was achieved. An apparent density d=1.13 g/ml in sucrose and a Sw 20 value of about 150 was determined for purified HC virions.

This publication has 30 references indexed in Scilit: