Studies on the Interaction between Leukocyte Elastase, Antileukoproteinase and the Plasma Proteinase Inhibitors α1-Proteinase Inhibitor and α2-Macroglobulin
- 1 January 1983
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 364 (2) , 793-800
- https://doi.org/10.1515/bchm2.1983.364.2.793
Abstract
The dominating inhibitor of leukocyte elastase in human respiratory tract secretions is a low molecular mass inhibitor, designated anti-leukoproteinase. An equimolar antileukoproteinase-elastase complex was produced and subjected to gel filtration after differing time intervals and was found to be stable. On addition to human serum, however, elastase dissociated from antileukoproteinase and formed a complex with .alpha.1-proteinase inhibitor. A small amount of elastase was also found bound to .alpha.2-macroglobulin. Antileukoproteinase was capable of inhibiting elastase bound to .alpha.2-macroglobulin. This inhibition was more complete and more rapid when the .alpha.2-macroglobulin-elastase complex was in a molar ratio of 1:1 than in a ratio of 1:2.This publication has 15 references indexed in Scilit:
- Distribution of Antileukoprotease in Upper Respiratory MucosaAnnals of Otology, Rhinology & Laryngology, 1982
- Kinetics of the inhibition of leukocyte elastase by the bronchial inhibitorBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Glutaminyl-Peptide γ-Glutamyltransferase Dependence of the Uptake of Trypsin-α-Macroglobulin Complexes by MacrophagesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1982
- Studies on the pancreatic secretory trypsin inhibitor in plasma and its complex with trypsin in vivo and in vitroScandinavian Journal of Clinical and Laboratory Investigation, 1978
- The Induction of Pulmonary Emphysema with Human Leukocyte Elastase1–3American Review of Respiratory Disease, 1977
- Isolation and Partial Characterization of a Low Molecular Weight Acid Stable Protease Inhibitor from Human Bronchial SecretionHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- Human alpha-1-proteinase inhibitor mechanism of action: Evidence for activation by limited proteolysisBiochemical and Biophysical Research Communications, 1976
- Inhibition of Elastase from Granulocytes by the Low Molecular Weight Bronchial Protease InhibitorScandinavian Journal of Clinical and Laboratory Investigation, 1976
- Granulocyte Collagenase, Elastase and Plasma Protease Inhibitors in Purulent SputumEuropean Journal of Clinical Investigation, 1975
- Electroimmuno AssayScandinavian Journal of Clinical and Laboratory Investigation, 1972