Mass spectrometry: A tool for the identification of proteins separated by gels
- 1 June 2000
- journal article
- review article
- Published by Wiley in Electrophoresis
- Vol. 21 (11) , 2105-2114
- https://doi.org/10.1002/1522-2683(20000601)21:11<2105::aid-elps2105>3.0.co;2-m
Abstract
Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high-resolution two-dimensional (2-D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments during the last five years in MS associated with protein gel separation are reviewed.Keywords
This publication has 100 references indexed in Scilit:
- A dynamic two-dimensional polyacrylamide gel electrophoresis database: The mycobacterial proteomevia InternetElectrophoresis, 1999
- High-throughput mass spectrometric discovery of protein post-translational modificationsJournal of Molecular Biology, 1999
- Analytical techniques used to study the degradation of proteins and peptides: chemical instabilityJournal of Pharmaceutical and Biomedical Analysis, 1998
- Protein identification with N and C-terminal sequence tags in proteome projectsJournal of Molecular Biology, 1998
- Electrophoresis‐related protein modification: Alkylation of carboxy residues revealed by mass spectrometryElectrophoresis, 1998
- Optimization of capillary chromatography ion trap-mass spectrometry for identification of gel-separated proteinsElectrophoresis, 1998
- A strategy for the identification of proteins localized to subcellular spaces: Application to E. coli periplasmic proteinsInternational Journal of Mass Spectrometry and Ion Processes, 1997
- Two‐dimensional electrophoretic analysis of human breast carcinoma proteins: Mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2Electrophoresis, 1997
- cDNA expression and human two-dimensional gel protein databases: Towards integrating DNA and protein informationElectrophoresis, 1996
- Surfaces for interfacing protein gel electrophoresis directly with mass spectrometryMass Spectrometry Reviews, 1995