Thiourea, a Growth Promoter of Callus Tissues1

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Abstract
Thiourea (TU) promoted growth in cytokinin-requiring soybean, tobacco, and apple callus tissues, in the absence of kinetin. The increase in fresh and dry weight obtained was a result of cell multiplication. Callus tissues grew well when repeatedly subcultured in kinetin-free medium containing TU. The best growth was obtained with 10−4-10−3 M TU. At these concentrations synergism was demonstrated between TU and the cytokinins, kinetin, benzyladenine, and zeatin. TU caused greater growth than diphenylurea and phenylthiourea. The possibility that a breakdown product of TU is responsible for the enhanced growth was not excluded. The dormancy-breaking effect of TU was suggested to be related to its growth-enhancing effect.

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