Detection of Gangliosides as N‐Glycolylneuraminic Acid‐specific Tumor‐associated Hanganutziu‐Deicher Antigen in Human Retinoblastoma Cells

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Abstract
Gangliosides were shown to bear the tumor‐associated N‐glycolylneuraminic acid (NeuGc)‐specific Hanganutziu‐Deicher (HD) antigen expressed in human retinoblastoma cells. HD antigenie gangliosides were detected by thin‐layer chromatography/enzyme‐immunostaining using affinity‐purified chicken antibody against GM3 containing NeuGc and horseradish peroxidase‐conjugated anti‐chicken IgG. One to four species of the antigenic gangliosides were detected from all of 4 cell lines, Y79, WERI‐Rb1, TOTL1, and YK, as well as freshly cultured retinoblastoma cells and isolated tumor tissue. All cases contained GMS(NeuGc) as an HD antigen. No HD antigenic ganglioside was detected in normal retinal tissues by the same procedure.