Effective Cloning of Unmarked DNA Fragments in theBacillus subtilis168 Genome
- 1 January 1999
- journal article
- Published by Oxford University Press (OUP) in Bioscience, Biotechnology, and Biochemistry
- Vol. 63 (3) , 602-604
- https://doi.org/10.1271/bbb.63.602
Abstract
The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.Keywords
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