EFFECT OF VARIOUS CARBON SOURCES ON GROWTH OF SCLEROTINIA TRIFOLIORUM

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Abstract
Three isolates of Sclerotinia trifoliorum Erikss. grew well in stationary culture in a synthetic liquid medium containing DL-asparagine, mineral salts, and a number of carbon sources representing monosaccharides, oligosaccharides, polysaccharides, and carbohydrate derivatives. D-Glucose, D-galactose, D-mannose, sucrose, lactose, dextrin, starch, and glycogen were the best carbon sources. D-Fructose, maltose, cellobiose, raffinose, and inulin supported considerable growth but somewhat less than the previous group. All other sources were utilized only slightly for growth during a 3-week incubation period. Significant differences among the isolates were observed on a number of carbon sources.L-Sorbose was utilized only slightly for growth during an 8-week incubation. When L-sorbose contributed one-half of the carbon in mixed media, growth was inhibited for 4 weeks in the presence of D-glucose and for the duration of the test period (8 weeks) in the presence of sucrose and maltose. Other monosaccharides, not utilized for growth when present alone in the medium, did not inhibit growth when in the presence of D-glucose. When the proportion of carbon contributed by L-sorbose was reduced to one-third, inhibition of growth was decreased.

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