Quantitative Analysis of Dammarane Type Saponins of Ginseng and Its Application to the Evaluation of the Commercial Ginseng Tea and Ginseng Extract

Abstract
On hydrolysis with dilute mineral acid, ginseng saponins, ginsenosides-Rb1, -Rb2, -Rb3, -Rc, and Rd yielded panaxadiol (IV), and ginsenosides-Re, -Rf, -Rg1, and Rg2 and 20-glucoginsenoside-Rf afforded panaxatriol (X). The optimal condition of saponin hydrolysis for the quantitative analysis of IV and X was studied. The crude hydrolysates of methanol extract of ginseng were trimethylsilylated with N-trimethylsilylimidazole and the saponin content was calibrated by the gas chromatographic determination of TMS-panaxadiol and TMS-panaxatriol, using diacetylhederagenin methyl ester as the internal standard. This procedure was applied to the evaluation of the commercial ginseng tea and ginseng extract.