Human Immunodeficiency Virus Type 1 (HIV-1) Antigen Secretion by Latently Infected Resting CD4 + T Lymphocytes from HIV-1-Infected Individuals

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Abstract
In resting CD4 + T lymphocytes harboring human immunodeficiency virus type 1 (HIV-1), replication-competent virus persists in patients responding to highly active antiretroviral therapy (HAART). This small latent reservoir represents between 10 3 and 10 7 cells per patient. However, the efficiency of HIV-1 DNA-positive resting CD4 + T cells in converting to HIV-1-antigen-secreting cells (HIV-1-Ag-SCs) after in vitro CD4 + -T-cell polyclonal stimulation has not been satisfactorily evaluated. By using an HIV-1-antigen enzyme-linked immunospot assay, 8 HIV-1-Ag-SCs per 10 6 CD4 + resting T cells were quantified in 25 patients with a plasma viral load of 6 CD4 + T cells were enumerated in the two groups of patients, respectively. Only a minority of latently HIV-1 DNA-infected CD4 + T cells could be stimulated in vitro to become HIV-1-Ag-SCs, particularly in aviremic patients. The difference between the number of HIV-1 immunospots in viremic versus aviremic patients could be explained by HIV-1 unintegrated viral DNA that gave additional HIV-1-Ag-SCs after in vitro CD4 + -T-cell polyclonal stimulation. The ELISPOT approach to targeting the HIV-1-Ag-SCs could be a useful method for identifying latently HIV-1-infected CD4 + T cells carrying replication-competent HIV-1 in patients responding to HAART.