Kininogenase Activity in Rat Uterus Homogenates

Abstract
A synthetic peptide substrate for tissue kallikreins was hydrolyzed by uterus homogenates. These also released kinins when incubated with dog kininogen. By using high pressure liquid chromatography in combination with a radioimmunoassay for kinins, the peptides released were identified as bradykinin and kallidin. Plasma kallikrein and trypsin released bradykinin from dog kininogen and hog pancreas kallikrein released kallidin from this substrate. A tissue kallikrein and also a trypsin-like enzyme or perhaps plasma kallikrein are present in rat uterus.

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