High Throughput Screening Technologies for Direct Cyclic AMP Measurement
- 1 April 2003
- journal article
- research article
- Published by Mary Ann Liebert Inc in ASSAY and Drug Development Technologies
- Vol. 1 (2) , 291-303
- https://doi.org/10.1089/15406580360545107
Abstract
A study comparing five different cAMP detection technologies in terms of sensitivity, robustness, and feasibility for HTS is presented. In this report, the following methods are described: a nonhomogeneous DELFIA, and the homogeneous methods based on time-resolved fluorescence (HTRF), luminescent singlet oxygen channeling or ALPHAScreen, FP, and high-affinity enzyme complementation. DELFIA had the highest sensitivity, whereas ALPHAScreen and HTRF shared several advantages, including high sensitivity, broad dynamic range, and minimal reagent addition steps. For G(s)-coupled antagonist screens, we found HTRF and ALPHAScreen the more sensitive and HTS-compatible techniques.Keywords
This publication has 18 references indexed in Scilit:
- A Homogeneous Enzyme Fragment Complementation Cyclic AMP Screen for GPCR AgonistsSLAS Discovery, 2002
- Enzyme Fragment Complementation: A Flexible High Throughput Screening Assay TechnologyASSAY and Drug Development Technologies, 2002
- Patent status of the therapeutically important G-protein-coupled receptorsExpert Opinion on Therapeutic Patents, 2001
- Homogeneous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreenGenome Research, 2001
- DRUG ANALYSIS BASED ON SIGNALING RESPONSES TO G-PROTEIN-COUPLED RECEPTORSJournal of Receptors and Signal Transduction, 2001
- Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practiceDrug Discovery Today, 1999
- A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening AssaysSLAS Discovery, 1999
- Time-resolved fluorometry: an overview of the labels and core technologies for drug screening applicationsDrug Discovery Today, 1997
- Selection of an Optimal Reporter Gene for Cell-Based High Throughput Screening AssaysSLAS Discovery, 1997
- Use of a luciferase reporter system for characterizing G-protein-linked receptorsCurrent Opinion in Biotechnology, 1995