Slide method for detection of antibody-coated bacteria in urine sediments

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Abstract
An immunofluorescent slide method incorporating 0.1% (wt/vol) Evans blue as a counterstain was developed and compared with a recently described tube method. Seventy-one [human] urine specimens were tested concurrently by both methods. Results of the 2 methods agreed in 70 specimens and disagreed in only 1. The slide method is less costly and time-consuming than the tube method.