A Sensitive and Quantitative Element-Tagged Immunoassay with ICPMS Detection
- 22 February 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 74 (7) , 1629-1636
- https://doi.org/10.1021/ac0110350
Abstract
We report a set of novel immunoassays in which proteins of interest can be detected using specific element-tagged antibodies. These immunoassays are directly coupled with an inductively coupled plasma mass spectrometer (ICPMS) to quantify the elemental (in this work, metal) component of the reacted tagged antibodies. It is demonstrated that these methods can detect levels of target proteins as low as 0.1−0.5 ng/mL and yield a linear response to protein concentration over 3 orders of magnitude.Keywords
This publication has 8 references indexed in Scilit:
- Nanosphere-antibody conjugates with releasable fluorescent probes.Analytical and Bioanalytical Chemistry, 2001
- Worth Its Weight in GoldScience, 2001
- Electrothermal Atomic Absorption Spectrometric Diagnosis of Familial HypercholesterolemiaAnalytical Chemistry, 2000
- Giant Platinum Clusters: 2-nm Covalent Metal Cluster LabelsJournal of Structural Biology, 1999
- Recent advances in the determination of the platinum group elements and goldTalanta, 1999
- A Rapid Procedure Suitable to Assess Quantitatively the Endocytosis of Colloidal Gold and Its Conjugates in Cultured CellsJournal of Histochemistry & Cytochemistry, 1998
- Characterization of ionization and matrix suppression in inductively coupled ‘cold’ plasma mass spectrometryJournal of Analytical Atomic Spectrometry, 1995
- A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling.Journal of Histochemistry & Cytochemistry, 1992