Impaired CD28-mediated co-stimulation in anergic T cells

Abstract
We have investigated a CD28 co-stimulation in anergic T cells in staphylococcal enterotoxin Binoculated mice by stimulating the cells with a plate-coated anti-TCR antibody in the presence or absence of an anti-CD28 antibody. CD28 co-stimulation increased the levels of IL-2 and IL-4 mRNAs in nalve CD4+Vβ8+ T cells. However, it did not increase the levels of IL-4 mRNA at all and only partially increased those of IL-2 mRNA in anergic T cells. It was demonstrated that CD28 co-stimulation was impaired so that it no longer stabilized cytoklne mRNAs in anergic cells. The levels of IL-4 mRNA in response to TCR stimulation were higher in anergic T cells than those in nalve T cells in spite of the defective CD28 co-stimulation in the former cells. Anergy induction and generation of a Th2-type immune response in vivo are discussed

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