A New Method for the Purification of DNA‐Binding Proteins with Sequence Specificity
- 1 February 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 104 (1) , 5-11
- https://doi.org/10.1111/j.1432-1033.1980.tb04392.x
Abstract
A technique is described for a rapid and efficient isolation and purification of proteins binding to defined DNA sequences. Cloned double-stranded DNA was covalently coupled to m-aminobenzyloximethylcellulose to purify proteins which recognize and bind to specific sequences on the DNA. The purification of 2 DNA-binding proteins from Drosophila melanogaster is demonstrated using the respective cloned DNA sequences.This publication has 39 references indexed in Scilit:
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