PREPARATION OF COMPLEMENT FIXING ANTIGEN FROM CRYPTOCOCCUS NEOFORMANS: PRELIMINARY REPORT

1 June 1955

journal article
research article
Published by Canadian Science Publishing in Canadian Journal of Microbiology

Vol. 1 (6) , 451-454
https://doi.org/10.1139/m55-056

Abstract

Complement fixing antigen from Cryptococcus neoformans strains was prepared by treating the organisms with pyridine for two hours at room temperature, washing three times with buffered 0.85% sodium chloride solution, resuspending, and subsequently exposing the suspension to ultrasonic vibration for 10 min. The antigen so prepared is not anticomplementary and appears to be specific and sensitive.

Keywords

COMPLEMENT
CRYPTOCOCCUS