Sequential distribution of the mycotoxin deoxynivalenol in wheat spikes after inoculation with Fusarium graminearum

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Abstract
One central spikelet of spring wheat (Triticum aestivum) cv. Roblin spikes was inoculated with macroconidia of Fusarium graminearum and the entire spikes were harvested at 2- to 4-day intervals from 2 to 25 days after inoculation. The spikes were dissected and the amount of deoxynivalenol (DON) in each spikelet and in each internode of the rachis was measured by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. High concentrations of DON were first detected in the inoculated spikelets, 4 days after inoculation. DON concentrations in the spikelets below the inoculation point eventually reached 500-600 ppm while the corresponding internodes of the rachis contained 1000-1200 ppm. Much lower amounts of DON were found in spikelets and rachis above the inoculation point.