DECREASE OF LIVER PROTEIN-KINASE-C IN STREPTOZOTOCIN-INDUCED DIABETIC RATS AND RESTORATION BY VANADATE TREATMENT

  • 1 January 1990
    • journal article
    • research article
    • Vol. 21  (4) , 651-657
Abstract
Effects of streptozotocin-induced diabetes and administration of the insulin-mimetic agent, vanadate in rats on the liver protien kinase C-induced phosphorylation of exogenous C (Histone III-S) and endogenous substrates were investigated. Diabetes caused a significant fall (40-60%) in liver cytosolic protein C activity measured using both types of substrates. Vanadate treatment for a period of 5 weeks restored them to normal levels. Phosphorylation of cytosolic target proteins for protein kinase C followed a similar pattern in response to diabetes and vanadate. These treatments had no effect on particulate protein kinase C activity. Vanadate also had no effect in normal livers with respect to the protein kinase C system.

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