Cell surface distribution of lectin receptors determined by resonance energy transfer

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Abstract
The surface topography of concanavalin A (con A) bound to normal [3T3 embryo] and [SV40] transformed [SV3T3] murine fibroblasts was studied by a new technique involving fluorescence resonance energy transfer (RET). RET can provide a high resolution map of the distances separating con A-receptor complexes in single living cells. The distribution of con A is non-random in normal and transformed cells, but sites are more closely approximated in the transformed cell. Approximation is induced by the con A but occurs at extremely slow rates, indicating that the topography is not primarily determined by simple diffusion of complexes.