Combined immunostaining and Coomassie Brilliant Blue staining of polyvinylidene difluoride membranes without organic solvent
- 1 January 1997
- journal article
- Published by Wiley in Electrophoresis
- Vol. 18 (5) , 701-705
- https://doi.org/10.1002/elps.1150180508
Abstract
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of the membrane is needed afterwards. Preblocking with polyethylene glycol is compatible with microsequencing while Tween 20 leads to very low initial yields. Preblocking with Tween 20 has the additional advantage of allowing immunostaining followed by Coomassie Brilliant Blue staining for total protein on the same membrane.Keywords
This publication has 7 references indexed in Scilit:
- Comparison of different staining methods for polyvinylidene difluoride membranesElectrophoresis, 1992
- Determination of amino acid compositions and NH2-terminal sequences of peptides electroblotted onto PVDF membranes from Tricine-Sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Application to peptide mapping of human complement component C3Analytical Biochemistry, 1989
- Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membraneBiochemistry, 1988
- Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.Journal of Biological Chemistry, 1987
- Protein blottingElectrophoresis, 1986
- Protein blotting: Principles and applicationsAnalytical Biochemistry, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970