[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].

  • 1 February 1996
    • journal article
    • abstracts
    • Vol. 76  (2) , 96-9
Abstract
To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells. The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate. Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining. In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.

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