Helicobacter pyloriModulates the T Helper Cell 1/T Helper Cell 2 Balance through Phase-variable Interaction between Lipopolysaccharide and DC-SIGN
Open Access
- 18 October 2004
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 200 (8) , 979-990
- https://doi.org/10.1084/jem.20041061
Abstract
The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. pylori variants are able to bind to the C-type lectin DC-SIGN and present on gastric dendritic cells (DCs), and demonstrate that this interaction blocks T helper cell (Th)1 development. In contrast, Le− variants escape binding to DCs and induce a strong Th1 cell response. In addition, in gastric biopsies challenged ex vivo with Le+ variants that bind DC-SIGN, interleukin 6 production is decreased, indicative of increased immune suppression. Our data indicate a role for LPS phase variation and Le antigen expression by H. pylori in suppressing immune responses through DC-SIGN.Keywords
This publication has 63 references indexed in Scilit:
- Antigenic Properties of HpaA and Omp18, Two Outer Membrane Proteins ofHelicobacter pyloriInfection and Immunity, 2003
- Interactions of the gastrotropic bacteriumHelicobacter pyloriwith the leukocyte–endothelium adhesion molecules, the selectins – a preliminary reportFEMS Immunology & Medical Microbiology, 2003
- The dendritic cell-specific C-type lectin DC-SIGN is a receptor for Schistosoma mansoni egg antigens and recognizes the glycan antigen Lewis xGlycobiology, 2003
- Identification of Different Binding Sites in the Dendritic Cell-specific Receptor DC-SIGN for Intercellular Adhesion Molecule 3 and HIV-1Journal of Biological Chemistry, 2002
- Helicobacter pyloriInfection and the Development of Gastric CancerNew England Journal of Medicine, 2001
- Lipopolysaccharides of Helicobacter Pylori Serogroups O:3 and O:6 Structures of a Class of Lipopolysaccharides with Reference to the Location of Oligomeric Units of D‐Glycero‐α‐D‐Manno‐Heptose ResiduesEuropean Journal of Biochemistry, 1997
- Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomyMicrobiology, 1996
- Variation of gonococcal lipooligosaccharide structure is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases.The Journal of Experimental Medicine, 1996
- Phenotypic variation of carbohydrate surface antigens and the pathogenesis of Haemophilus influenzae infectionsTrends in Microbiology, 1995
- Molecular mechanisms and implications for infection of lipopolysaccharide variation in NeisseriaMolecular Microbiology, 1995