Pollen-Derived Haploids ofNicotiana Knightiana, N. raimondii,andN. attenuata

1 December 1974

journal article
research article
Published by Oxford University Press (OUP) in Journal of Experimental Botany

Vol. 25 (6) , 1030-1039
https://doi.org/10.1093/jxb/25.6.1030

Abstract

Haploid plantlets were obtained in large numbers in three diploid, 24-chromosome species of Nicotiana by culture of anthers at or just past the first pollen mitosis. The three species were N. Knightiana, N. raimondii, and N. attentiata. Efficiency of haploid production varied from about 10 per cent in N. attenuata cultures to 30 and 38 per cent respectively in cultures of N. raimondii and N. Knightiana. H-medium without hormones and standard cultural conditions were used. N. Knightiana appeared to be especially suitable for haploid studies on account of its high plantlet productivity, low chromosome number, and distinctive karyotype.

Keywords

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