DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: V. INHIBITORS OF TRYPSIN FROM GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE)

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Abstract
Trypsin has been partially purified from the midguts of Glossina morsitans. It is inactivated by tosyl-l-lysine chloromethyl ketone, phenylmethane sulfonylfluoride, and 2-mercaptoethanol. Mammalian sera inhibit G. morsitans trypsin in vitro. Sheep serum contains at least two inhibitors of G. morsitans trypsin and a high molecular weight material which protects against serum inhibition. Very low levels of trypsin inhibitor are found in G. morsitans primary excreta and in the salivary glands, Malpighian tubules, and anterior midguts of unfed flies. High concentrations of trypsin inhibitor are found in the anterior (non-digestive) section of the midgut for at least 48 h after flies fed on goats.