Accumulation of polyploid cells and G2‐phase cells during ascites tumor growth

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Abstract
Ehrlich ascites tumor cells from the plateau phase of growth were transplanted into new hosts, pulse‐labeled with tritiated thymidine and blocked with repeated injections of vinblastine. When unlabeled cells were analyzed for their cellular DNA content utilizing a cytophotometric technique it was found that in relation to the total number of cells (labeled plus unlabeled), 13% had a 2C DNA content, 36% a 4C DNA content and 5% an 8C DNA content at 0.5 hours after transplantation. By 24 hours the distributions changed dramatically: the initially unlabeled 2C cells were now 4C, the 36% of the cells that were initially 4C partitioned into 24% that were still 4C and 12% that progressed to 8C, and the initial 8C cells remained 8C. These studies indicate that the accumulation of 4C cells during the plateau phase of growth is due to a combination of G2 diploid and G1 tetraploid cells.