A sensitive assay of 5‐fluorouracil in plasma by gas chromatography‐ mass spectrometry.

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Abstract
1 A gas chromatographic‐mass spectrometric method was developed for determining 5‐fluorouracil in plasma, using methylated thymine as an internal standard. 2 5‐fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5‐fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.