Identification of the yeast methionine biosynthetic genes that require the centromere binding factor 1 for their transcriptional activation
- 19 June 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 367 (1) , 15-18
- https://doi.org/10.1016/0014-5793(95)00528-h
Abstract
The yeast Centromere binding factor I (Cbf1) belongs to the family of the DNA binding factors that recognize the consensus sequence CACGTG. Phenotypic studies of cells lacking Cbf1 revealed that this factor is actually involved in two cellular processes; the fidelity of the chromosomal segregation and the metabolism of sulfur amino acids. However, the function of Cbf1 in the regulation of the sulfur amino acid metabolism is now a matter of controversy in literature with conflicting reports about its binding to the CACGTG sequences found upstream to the methionine biosynthetic genes. To provide a reliable basis for the functional analysis of Cbf1, we present an analysis of the transcription of the methionine biosynthesic genes in cells lacking Cbf1. Our results prove that Cbf1 is indeed involved in the transcriptional regulation of the sulfur amino acid metabolism.Keywords
This publication has 24 references indexed in Scilit:
- A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coliPublished by Elsevier ,2003
- The synthesis of the two S-adenosyl-methionine synthetases is differently regulated in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1991
- DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeastNucleic Acids Research, 1991
- Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1.Molecular and Cellular Biology, 1990
- Yeast centromere binding protein CBF1, of the helix-loop-helix protein family, is required for chromosome stability and methionine prototrophyCell, 1990
- Elements involved in S-adenosylmethionine-mediated regulation of the Saccharomyces cerevisiae MET25 gene.Molecular and Cellular Biology, 1989
- Mutational analysis of centromere DNA from chromosome VI of Saccharomyces cerevisiae.Molecular and Cellular Biology, 1988
- The Saccharomyces cerevisiae MET3 gene: Nucleotide sequence and relationship of the 5′ non-coding region to that of MET25Molecular Genetics and Genomics, 1987
- Nucleotide sequence of theSaccharomyces cerevisiaeMET25 geneNucleic Acids Research, 1986