Relationship between Vital Staining and Subculture Growth during the Senescence of Plant Tissue Cultures
Open Access
- 1 October 1982
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 70 (4) , 1228-1230
- https://doi.org/10.1104/pp.70.4.1228
Abstract
The vital staining properties of rose cultures (Rosa cv Paul's Scarlet) of increasing age were compared with their ability to be subcultured. At 4-day intervals beginning on day 14, after cell division and expansion had stopped, cells were stained separately with Evans blue, fluorescein diacetate, and phenosafranine. The degree to which parent cultures stained with each of these dyes was compared to the dry weight of their subcultures harvested after 9 and 21 days of growth.Keywords
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