INHIBITION OF YEAST METABOLISM BY 2-DEOXYGLUCOSE, 6-DEOXY-6-FLUOROGLUCOSE AND THE CORRESPONDING GALACTOSE DERIVATIVES

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Abstract
The inhibitory effects of 2-deoxy-D-glucose (2DG) and 6-deoxy-6-fluoro-D-glucose (6FG) on the metabolism of glucose by yeast were compared and some properties of the glucose transport system were examined. 2DG and 6FG had similar effects on the consumption of oxygen and the production of carbon dioxide by several strains of yeast, hence they appear to inhibit metabolism of glucose by the same mechanism. Kinetic studies with mixed inhibitors indicated that both compounds compete at the same catalytic site. The rate of entry of 6FG into the yeast cell and its equilibrium concentration were similar to those of galactose. The fermentation of D-galactose was strongly inhibited by 2DG and 6FG, and weakly inhibited by 2-deoxy-D-galactose and 6-deoxy-6-fluoro-D-galactose. The latter two compounds did not inhibit the fermentation of D-glucose.