In vitro incorporation of L-canavanine into vitellogenin of the fat body of the migratory locust Locusta migratoria migratorioides.
- 1 September 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (9) , 5480-5483
- https://doi.org/10.1073/pnas.78.9.5480
Abstract
L-Canavanine competes with L-arginine for incorporation into vitellogenin secreted in vitro by the fat body of the female locust L. migratoria migratorioides. Incorporation of L-[guanidinooxy-14C]canavanine into vitellogen was established by combined arginase and urease hydrolyses of the acid hydrolysate of antibody-precipitated canavanyl vitellogenin. Continued exposure of the fat body to canavanine decreases in vitro protein secretion but the proportion of canavanyl vitellogenin to native vitellogenin increases. Conavanine-mediated inhibition of faty body protein secretion is dependent on both the canavanine concentration and the arginine retention of the fat body. Conavanine replaces about 10% of the arginyl residues of canavanyl vitellogenin. The electrophoretic mobility of canavanyl vitellogenin is greater than that of native vitellogenin, but the ability of this aberrant portein to react with vitellogenin antibody is unimpaired.This publication has 15 references indexed in Scilit:
- Inhibition of protein secretion and protein kinase activity in the locust fat body by diamide (Azodicarboxylic acid-bis-dimethylamide)Archives of Biochemistry and Biophysics, 1978
- Preparation and colorimetric analysis of l-CanavanineAnalytical Biochemistry, 1977
- Intracellular Protein Degradation in Mammalian and Bacterial Cells: Part 2Annual Review of Biochemistry, 1976
- Additive and Synergistic Growth-inhibiting Properties of the Canaline-Urea Cycle Amino AcidsPlant Physiology, 1976
- Non-protein amino acid-insect interactions—II. Effects of canaline-urea cycle amino acids on growth and development of the tobacco hornworm, Manduca Sexta L. (Sphingidae)Comparative Biochemistry and Physiology Part A: Physiology, 1975
- Studies on the Growth Effects of the Canaline-Urea Cycle Amino Acids with Lemna minor L.Plant Physiology, 1975
- Non-protein amino acid-insect interactions—I. Growth effects and symptomology of l-canavanine consumption by tobacco hornworm, Manduca sexta (L.)Comparative Biochemistry and Physiology Part A: Physiology, 1975
- Intracellular Protein Degradation in Mammalian and Bacterial CellsAnnual Review of Biochemistry, 1974
- Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatographyAnalytical Biochemistry, 1966
- Purification and Substrate Specificity of Arginyl-Ribonucleic Acid Synthetase from Rat LiverJournal of Biological Chemistry, 1964